Genetic circularity of the Pseudomonas aeruginosa PAO chromosome
نویسندگان
چکیده
منابع مشابه
A combined physical and genetic map of Pseudomonas aeruginosa PAO.
A combined physical and genetic map of Pseudomonas aeruginosa PAO was constructed by pulsed-field gel electrophoresis and Southern hybridization using cosmid clones from a genomic library carrying known genes. A total of 37 SpeI restriction fragments have been mapped on the 5862 kb genome, and fragment contiguity demonstrated by hybridization with clones from a SpeI junction fragment library an...
متن کاملIdentification of an insertion sequence in the chromosome of Pseudomonas aeruginosa PAO.
An insertion sequence, IS22, in Pseudomonas aeruginosa PAO was identified by its genetic properties. It occurred as a single copy in the chromosome, as shown by Southern hybridization. A restriction map of it was constructed.
متن کاملInducible UV repair potential of Pseudomonas aeruginosa PAO.
Pseudomonas aeruginosa PAO lacks UV-inducible Weigle reactivation and Weigle mutagenesis of UV-damaged bacteriophages. This lack of UV-inducible, error-prone DNA repair appears to be due to the absence of efficiently expressed umuDC-like genes in this species. When the P. aeruginosa recA gene is introduced into a recA(Def) mutant of Escherichia coli K12, the P. aeruginosa recA gene product is c...
متن کاملNovel pyoverdine biosynthesis gene(s) of Pseudomonas aeruginosa PAO.
Conjugational mobilization of a Pseudomonas aeruginosa PAO1 cosmid bank (in pMMB33) into a pyoverdine-deficient (pvd) mutant harbouring a mutation in the 47 min region of the chromosome yielded one clone which restored yellow-green pigmentation and fluorescence when grown on iron-deficient medium. The relevant pMMB33-derivative cosmid, pPYP17, contained a 15.1 kb insert which was subcloned into...
متن کاملDevelopment of broad-host-range vectors and gene banks: self-cloning of the Pseudomonas aeruginosa PAO chromosome.
A host-vector system for Pseudomonas aeruginosa PAO was developed. Scattered regions of the strain PAO chromosome were cloned by direct selection for complementation of auxotrophs or from a DNA gene bank which contains over 1,000 independently isolated chromosome-vector recombinant plasmids. The use of partially digested chromosomal DNA facilitated the selection of a variety of strain PAO chrom...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1981
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.145.1.145-155.1981